Other
Part:BBa_K648106:Design
Designed by: Alex Bina Group: iGEM11_Penn_State (2011-10-23)
RecA1 with mutation of Arg243
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 127
Illegal AgeI site found at 631 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Remove restriction sites and one source of recombinant activity.
Source
genome sequence from DH10B strain of Escherichia coli bacteria.